exitium wrote:Im just curious, had some basic labs done a couple weeks ago. Non fasted glucose was high. I dont remember exactly but based on timing of my appt there is a very good chance I had 2-4 grams of ascorbate within an hour of my blood draw. Would this have any bearing on my blood glucose?
ofonorow wrote:exitium wrote:Im just curious, had some basic labs done a couple weeks ago. Non fasted glucose was high. I dont remember exactly but based on timing of my appt there is a very good chance I had 2-4 grams of ascorbate within an hour of my blood draw. Would this have any bearing on my blood glucose?
The answer is no.
However, did you just have a meal, or when was the last meal? Do you remember the numbers?
An expert I consulted said that the encapsulating liposome should not effect the glucose meter, it should be able to read the C encapsulated or not.
Emek Blair wrote:Dear Owen,
Is the pH of both solutions identical? I’m assuming that the LivOnLabs product is at a pH of around 7 while ours is a little acidic; electrochemical sensors such as glucose meters need to be calibrated for each pH. So you would need to adjust the pH of the solution to get your zero reading first and then use another solution to add our liposomes to.
I hope this helps.
The reading you are getting may be completely due to the pH change that occurs when adding our liposomes. The glucose meter is and electrochemical meter that is “dumb.” It can’t tell if the electrochemical potential at its surface is altered due to glucose or pH or other components in the formula such as flavor. All that the glucose meter knows is that the potential at its electrode surface has changed (please see schematic below). These electrodes are designed to only work at a neutral pH.
Electrode surface ______________________
Solution charge +-+-+-+-+-+-+-+-+-+-+-+-+-+-
Electrode surface ______________________
Solution charge +--+--+--+--+--+--+--+--+--+--
If you add baking soda then the ionic strength of the solution will go up which will cause other issues.
If you look up “Emek Blair” in google scholar you will see that I am an expert in enzyme electrochemistry especially using lipid systems on electrodes (the basis for the glucose meters) and have written book chapters, peer reviewed papers, and have received several awards for my work in that field.
This method of measurement needs to be controlled in several ways in order to get this experiment to work properly (pH, temperature, oxygen level, electrode area, and salt content….blood is very consistent in this way). This is due to the fact that as the pH changes, ionic strength changes, how the lipid will alter how the enzymes will interact with the electrode (this will actually enhance the signal…please see one of my papers http://pubs.acs.org/doi/abs/10.1021/ja0488333 as one of many examples of this situation). In this paper I used structured lipids to dramatically enhance the communication between the enzymes and the electrode…this is another potential reason that you are getting such a signal.
Now that I’m really thinking about this… There are probably about 10,000+ papers showing that structured lipids will enhance a signal at an electrode. Hence, I am more likely to support a statement that the glucose meter will read vitamin c more completely if structured lipids are used.
Please see figure 5 at http://citeseerx.ist.psu.edu/viewdoc/do ... 1&type=pdf. Without the structured lipids (in this paper it is a film) the enzymes are not communicating as well with the electrode. In figure 5, (a) is the blank, (b) is enzyme with structured lipids and (c) is enzyme without lipids. It is obvious that the enzyme without the structured lipid does not react as well.
We have two separate clinical studies showing the same thing, the blood levels of vitamin c are higher using our liposomes (one performed by Ph.D. clinical chemist as a paid consultant and the other by two professors at the University of Colorado as research partners). Our project at CSU goes far beyond just a bioavailability and is about basic scientific research.
Not to linger on this point but Figure 1 shows that their liposomes have the same effect on vitamin c blood levels as regular powdered vitamin c. As they show the blood level later on being increased (not liposomal vs powder) but in general. We reach about 350 uM/L using 5 grams of our liposomal vitamin c. They reached 400 uM/L using 20 g liposomal + 20 g powdered.
OWEN said: phospholipid choline
emek wrote:-(Empirical's) vitamin c test will break apart the liposome and measure the vitamin c encapsulated and not encapsulated. We have tested this using a technique called standard addition. The freezing test also does something a little different; when you initially stir in the liv on labs product, the lipids don’t allow the vitamin c to escape (this is due to viscosity) but during a freeze thaw cycle the whole solution goes through a phase change and allows the vitamin c to dissolve in the water and be measured.
The easiest way to break apart the liposomes is to dilute the volume dramatically and sonicate for 30 minutes. We already have stability data for the vitamin c in our liposomes. Our first round showed 1.2% depletion in 18 months using accelerated testing (45 C).
p.s. The sonication and large dilution together will destroy the liposomes. Sonication for the homemade “liposomes” does something a little different.
... human trials...
One week wash out period (control baseline)
Measure Vitamin C serum levels after fasting for 10 hours
Single 5 g Vitamin C dose as sodium ascorbate (liposomal)
Track ascorbate levels in blood serum for 10 hours after dose
We have 22 testimonies of curing/healing various illnesses within 4-8 hours. Illnesses such as tonsillitis, viral sore throat, coughs, colds, uvulitis, lowering type 2 diabetes blood sugar count etc etc.
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