Have tried the qualityliposomalc method of Liposomal VC production.
However accidentally allowed the bath temperature to exceed 40C - happened so quickly.
However that may have been fortuitous, if the transition temp of P.Choline is 41c.
In anycase tested it taking 30 grams/day in divided doses. Though it tasted acidic it produced no problems, except for when I upped a dose to 10 grams too close to a previous dose. So I guess accumulation residual Ascorbic Acid caused the - monetary diarrhea. In the end consumed the 161 grams without problem - except trying to swallow the stuff. I found drinking some cream first, to line the throat and esophagus and then washing down the product helped. The doses where bigger than you would normally take, but I was testing the liposomal nature of the process.
So the process must have worked fine if I could consume 161 grams of it with only one short bout of the runs.
Before I go on I want to make a note of the difference between the LivOn Lipid ingredient and Lecithin powder/granules we use.
Lecithin Powder – NOW brand contains
P.choline 46% .
P. Ethanolamine 20%
Which is a bit different from the granules makeup, which has one less component.
LivOn/Zhang Patent relies on P.Choline I believe.
The other lipids P.choline P.insotol P. Ethanolamine are also used in liposome creation, namely for cancer drug delivery. P.Choline was noted as being less stable, and a mixture of the lipids a better delivery mechanism for the cancer agent
Thus the mixture of lipids in the NOW powder may represent a better outcome for stable Liposome production. And the Powder maybe better than the granules since it contains one extra lipid variety.
This notation I found in one study regarding cancer drug Cisplatin.... Generally, liposomes are prepared from phosphatidylcholine (PC). However, liposomes consisting of 100% PC exhibit poor membrane fusion, cellular uptake and selective targeting capacities . To overcome these limitations, efforts have focused on preparing liposomes from mixtures of PC and other phospholipids, such as phosphatidylethanolamine (PE). ...
I note one of the other method mentioned before (Norma67) which I summarise underneath - BUT also another method mentioned in the LivOn/Zhang Patent - shown at Example 1 On page 12 here: https://patentimages.storage.googleapis ... 1280A1.pdf
Most of the methods don't vary too much, but may improve the percentage of encapsulation.
I will try both these methods shortly.Method Norma67
20 g lecithin - 0.8 g tocopherol - 16 g alcohol (ethanol 94°)
33 ml distilled water
2. Soak 4-12 hours – becomes milky
Room Temperature – separate container
20 g ascorbic acid - 110 ml distilled water
Heat Lecithin in bath 42-45C
Remove from bath
5. Add slowly with stirring
6. Shake in rotary fashion
Room temperature – becomes gel
Ultrasonic bath 30 minsMethod Zhang Patent
as Per Example 1 on Page 12. (seems pretty simple - but who knows)
1. Lecithin (phospholipon = P.choline) 97gm (18%) - Alcohol 98% 56.3gm - Room temperature
2. Separately (Na) Ascorbic Acid 109.5gm (22%) – water 251gm (49%) –EDTA 26.4mg – Room temperature pH 6.3
3. Add slowly Lecithin to (Na) Ascorbic Acid – frequent vortexing with mixer 15 minutes – Room temperature
4. Thicken to gel with additive - if desired.
Requires no heat, no sonication.
You can buy EDTA as a 'supplement'
EDTA is..https://en.wikipedia.org/wiki/Ethylened ... plications
VERBATIM from Patent - Example 1
Preparation of Sodium Ascorbate Entrapped Lipo SOS
0140. 97g Phospholipon50IP (a non-GMO vesicle-form
ing phosphatidylcholine lipid available from Lipoid) was
solubilized in 56.3 g 200 proof alcohol at room temperature.
Care was taken to ensure that all lipids were completely
solubilized and no undissolved lipid remained. 109.5 g. sodium ascorbate and 26.4 mg. EDTA was separately solubi
lized in 251 g reverse osmosis-treated water at room tempera
ture with normal agitation. The pH of the Naascorbate-EDTA
solution was adjusted to 6.3 The sodium ascorbate-EDTA
solution was filtered with a 0.2 um filter to remove dust
particles and other contaminants. Streams of the lipid solution
were slowly injected into the vitamin solution with continu
ous vortexing with a mixer. After all the lipid solution was
added, lipid hydration was allowed to continue about 15
minutes with frequent mixing. At this point, the liposomes
had formed in a smooth, translucent fluid.
0141 2.2 g xanthan gum (available from Sigma-Ald
rich R) and 1.38 g TweenTM 80 (available from Sigma Ald
rich R) were added to thicken the solution into a gel. The
resulting liposome solution was transparent with a honey-like