Telomere Timebomb

Discussion of the 2009 Noble Prize in Medicine, focusing on substances that reduce telomere shortening by activating the human telomerase enzyme

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Re: Telomere Timebomb

Post Number:#31  Post by ofonorow » Tue Dec 16, 2014 1:15 pm

Thank you tjohnson_nb. Excellent overview. Some points I am mulling over.

The connection between resveratrol and calorie-restriction was interesting!

I thought they may have confused the term "aging" with "life span" during the discussion of the antioxidant experiments.

The idea that the childhood aging disease progeria was not telomere shortening - but an abnormal protein was also interesting. Wonder if that has been confirmed.

The centenarians was an interesting segment, and in addition to their 3 genes, one wonders if their intracellular glutathione levels were higher - as per Levy's GSH MASTER DEFENDER revelations.
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Re: Telomere Timebomb

Post Number:#32  Post by ofonorow » Wed Dec 17, 2014 11:08 am

Another candidate to promote anti-aging/rejuvenation - AMKP enzyme stimulators.
http://www.lef.org/Magazine/2014/SS/AMPK/Page-01
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Re: Telomere Timebomb

Post Number:#33  Post by ofonorow » Wed Dec 17, 2014 11:57 am

https://www.youtube.com/watch?v=GzrMEgl2DZ0
Bruce Ames refers to Tomas Prolla's work. This video on calorie restriction - trying to understand it - is fascinating. Near the end they report the finding that intracellular glutathione (GSH) universally (all tissues) at least DOUBLES under calorie restriction.

leads to
Short-term consumption of a resveratrol-containing nutraceutical mixture mimics gene expression of long-term caloric restriction in mouse heart.
http://www.ncbi.nlm.nih.gov/pubmed/18657603

and to


Gene Expression Profiling of Aging in Multiple Mouse Strains: Identification of Aging Biomarkers and Impact of Dietary Antioxidants
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2733852/
We also tested the ability of dietary antioxidants to oppose these transcriptional markers of aging. Lycopene, resveratrol, acetyl-L-carnitine, and Tempol were as effective as caloric restriction in the heart, and α-lipoic acid and coenzyme Q10 were as effective as caloric restriction in the cerebellum. These findings suggest that transcriptional biomarkers of aging in mice can be used to estimate the efficacy of aging interventions on a tissue-specific basis.
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Re: Telomere Timebomb

Post Number:#34  Post by xor2 » Wed Dec 17, 2014 1:50 pm

What does this: HTLLAFB mean?

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Re: Telomere Timebomb

Post Number:#35  Post by tjohnson_nb » Wed Dec 17, 2014 3:26 pm

How To Live Longer .... by Pauling :)
'Always' and 'never' are 2 words you should always remember never to use.

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Re: Telomere Timebomb

Post Number:#36  Post by ofonorow » Thu Dec 18, 2014 10:51 am

Levy's reference on the study of oxidative stress (lower GSH) in those living to 100+

Oxidative stress and advancing age: results in healthy centenarians.
http://europepmc.org/abstract/med/9670869

and a study on resveratrol mimicing calorie restriction.
A Low Dose of Dietary Resveratrol Partially Mimics Caloric Restriction and Retards Aging Parameters in Mice
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0002264

Summary so far.

#1. For immortality (at least life-span increase) telemore shortening has to be dealt with. If telomerase preferentially lengthens the shortest telomeres, then it is key. (Human blood testing for report on the shortest telomers: http://lifelength.com If anyone finds cost/lab please post.)

#2 For Anti-Aging/Age reversal, calorie restriction is such a profound anti-aging mechanism, that it prevents age-related hearing loss - which can be experimentally measured in lab mice. No one wants to restrict calories so the search is on for what genes are expressed during calorie restriction. A laboratory set up to measure gene expression to measure whether drugs/supplements have an effect that mimics calorie restriction http://www.lifegentech.com

The team who set up this lab have duplicated Bruce Ames findings of Acetyl-L-Carnitine and Lipoic Acid (but also found Lycopene, CoQ10 and resveratrol also cause gene expression mimic'ing calorie restriction.)

#3 Vitamin C and perhaps especially Glutathione (GSH) is (are) intimately involved.

Dr. Levy found GSH research that a common denominator of people who live to be over 100 is high levels of glutathione in their cells. Yesterday, in the youtube video the Wisconsin team found that calorie restriction AT LEAST DOUBLES  glutathione levels in all tissues.

According to professor Emeritus of Chemistry Boyd Halley, high dose vitamin C is the only "proven" way to increase intracellular glutathione (GSH).

In the Bruce Ames ALC/ALA studies of these substances that can reverse aging in mice - a finding was increased levels of vitamin C in tissues.

We have at least two studies showing vitamin C reduces the shortening of telomeres, and one study in stem cells! Which reminds me.

Vitamin C Transforms Mouse Stem Cells Into Heart Muscle Cells
http://www.sciencedaily.com/releases/20 ... 073122.htm

Richard T. Lee, M.D., senior author of the study, says: "We have been taught for decades that when your heart cells are dead, they are dead and there is nothing we can do about it. We are excited about anything suggesting that we can grow more heart cells."

Lee and his colleagues tested 880 bioactive substances – including drugs and vitamins – approved by the U.S. Food and Drug Administration (FDA) to see if they stimulated the mouse stem cells to become heart muscle cells. The cells were genetically altered to give off a fluorescent bright green color when viewed under a microscrope if they had become heart muscle cells.

"We only got 1 out of the 880 to light up, and that was from ascorbic acid, the chemical commonly known as vitamin C," says Lee, an associate professor of medicine at Harvard Medical School and Brigham and Women's Hospital in Boston, and a lecturer in biological engineering at the Massachusetts Institute of Technology in Cambridge, Mass.


And finally, it is surprisingly difficult to raise intra-cellular vitamin C levels.
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Re: Telomere Timebomb

Post Number:#37  Post by ofonorow » Mon Dec 22, 2014 12:07 pm

First gene therapy successful against aging-associated decline: Mouse lifespan extended up to 24% with a single treatment
http://www.sciencedaily.com/releases/2012/05/120514204050.htm

In 2007, Blasco's group demonstrated that it was feasible to prolong the lives of transgenic mice, whose genome had been permanently altered at the embryonic stage, by causing their cells to express telomerase and, also, extra copies of cancer-­‐resistant genes. These animals live 40% longer than is normal and do not develop cancer.

The mice subjected to the gene therapy now under test are likewise free of cancer. ...

This study is viewed primarily as "a proof-­‐of-­‐principle that telomerase gene therapy is a feasible and generally safe approach to improve healthspan and treat disorders associated with short telomeres," state Virginia Boccardi (Second University of Naples) and Utz Herbig (New Jersey Medical School-­‐University Hospital Cancer Centre) in a commentary published in the same journal.
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Re: Telomere Timebomb

Post Number:#38  Post by ofonorow » Mon Dec 29, 2014 9:51 am

Scientists map elusive 3-D structure of telomerase enzyme, key actor in cancer, aging
http://newsroom.ucla.edu/releases/breakthrough-3-d-structure-of-245100
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Re: Telomere Timebomb

Post Number:#39  Post by ofonorow » Wed Dec 31, 2014 7:20 am

We have bitten the bullet :D We just placed the first order for what may very well be the world's first liposomal telomerase activator. The formula also contains molecules that have been shown to mimic calorie restriction's effect on genes and reverse aging in mice. In short, the unique liposomal formula is what I wanted for myself.

The bet is liposomal will work better than pills. The success of our first (China-FREE Quali-C) liposomal vitamin C has exceeded our wildest expectations. I am speaking of success against infections, i.e., the product works, and this has resulted in high sales volume. And as reported at this forum, the liposomal product has been picked up by Vitamin Shoppe as a new product for 2015.

Our experience with truly liposomal vitamin C has me more convinced that ever of Levy's contention, or empirical observation really, that clinically a truly encapsulated vitamin C exceeds the effectiveness of intravenous vitamin C directly into the vein. We are not sure why, but we know it isn't merely a matter of blood concentrations. It is our hope that the dosage amplification effect of delivering nutrients to the cytoplasm (and or nucleus) will allow the small (and thus less expensive dosages) to have a larger anti-aging and telomere lengthening effect than the pill form.

The first batch of the product will be labeled as an Inteligent*Vitamin*C product, but we may form a new company to produce and market follow-on batches. We are trying to find a way for early users to have their blood drawn and telomere length measured before and while taking the product. Ideally, Life Extension would offer the correct blood tests. We have communicated with LEF , but so far they seem uninterested. Another option is to try to work with the telomere measurement lab we have in mind, but we unsure how to obtain the “doctors order” script for the blood testing.

Since this first batch is a sort of Beta test, we will not be charging the full price, until we have established the efficacy of the product, so long as the consumer agrees to the blood tests. Therefore, early users must agree to blood testing for the discount, and perhaps sign an agreement with us. We expect that they will be able to purchase the product at least half the expected retail price of a proven product. We may have to adjust the formula in the future based on these initial measurements. (Note: We are hoping (and working) to be able to deliver the actual human telomerase enzyme itself in the future.)

If we are close to hitting a home run, imagine what kind of world we'd live in if Albert Eistein, Linus Pauling, Isaac Asimov and other geniuses were living and still contributing their combined knowledge and intelligence? Not to mention your favorite athletes extending their playing days into their fifth or six decade, etc

If you are willing to undergo blood testing, and would like to be an early adopter of the new liposomal telomerase activator, please call our 800 number and we'll add you to the wait list.

If you don't have any idea what we are talking about, then I suggest you read the book that is the title of this topic, Telomere Timebomb by Ed Park, and then the important follow-on book, Curing Aging by Bill Andrews.
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Re: Telomere Timebomb

Post Number:#40  Post by Johnwen » Wed Dec 31, 2014 10:50 am

Owen;

Here some links that may help for the testing.

http://telomeraseactivation.blogspot.co ... sting.html

http://www.telome.com/index.php

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3045570/

Some of these prices are high, but hay! they got to deal with spit in a cup! So I guess it's the high price of dealing with YUCK! :oops:
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Re: Telomere Timebomb

Post Number:#41  Post by ofonorow » Wed Dec 31, 2014 1:06 pm

Thank you johnwen - we'll take a look. Bill Andrews recommends lifelength.com - out of Spain - as they measure the shortest telomeres (under the theory we don't care as much about the longer ones?) We are in the process of contacting them. (Ouch thanks for those links. That is the first time we saw the price for lifelength - but if it is the only one to consider, the so be it.)

Added
And johnwen - we could use your web search capability (let me know if you want a bottle of this stuff). I found this paragraph in the last link particularly interesting

TA-65®, exclusively licensed to TA Sciences from Geron Corporation, is a >95% pure single chemical entity isolated from a proprietary extract of the dried root of Astragalus membranaceus and formulated into 5- to 10-mg capsules with inert excipients. Starting doses of 5–10 mg/day were considered safe on the basis of historical usage of extracts.



A Natural Product Telomerase Activator As Part of a Health Maintenance Program
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3045570/

We are making a huge assumption about what is inert and what isn't, in TA-65. Logically, given the expense of all three molecules lab tests have uncovered (that we now know about thanks to you!) Can you help us verify our assumption that the ingredient with the highest dosage (Cycloastragenol) is in fact the "single unique" molecule? (Very expensive filler if it isn't!)


I am irritated with Life Extension. Maybe there is something we are missing, something different about the telomere blood testing than their run-of-the-mill blood tests? For the record, here is there latest "rejection letter."

Dear Owen Fonorow,

Thank you for your recent correspondence.

Although Life Extension does not currently have plans to offer such a blood test, we do thank you for your suggestion and for your loyalty to health and longevity.

If there is anything else that we can help you with, please e-mail us or call the advisor helpline at (800) 226-2370; international customers dial 001-954-202-7660. We will be glad to assist you.

Thank you for contacting Life Extension during our annual Super Sale. We wish you a happy and healthy New Year.

Please make sure Case:[3200564] is in the subject line of all correspondence.

For Longer Life,
Life Extension
Health Advisors
(800) 226-2370


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Re: Telomere Timebomb

Post Number:#42  Post by Johnwen » Wed Dec 31, 2014 5:44 pm

It appears what their saying is that it don't slow aging but enhances Healthy Aging. Which isn't all that bad either!
I included Bill Andrews video (part 1 there is a link to Pt.2) of him talking about it. I didn't watch it probably tomorrow?? The other link gives an assessment of Ta-65 and the last one is the work Hf Valenzuela whose one of the author's of the previous article and his other works.

Hope this helps!

http://ta65review.org/dr-bill-andrews-o ... ocess-pt-1

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972662/

http://www.ncbi.nlm.nih.gov/pubmed?cmd= ... zuela%20HF[au]&dispmax=50
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Re: Telomere Timebomb

Post Number:#43  Post by ofonorow » Thu Jan 01, 2015 7:59 am

Terrific links, again thank you johnwen. What I think by what LEF initially told me, is that the telomere tests are so specialized that they required a doctor with knowledge of the results? In a way admitting that their staff can't handle the telomere measurement results. And perhaps there is some fear, maybe with the new Federal laws, of offering these tests.

They do call themselves "Life Extension" and could hire a physician who can evaluate the results, we would hope.

With this in mind, and the idea of "crowds" perhaps having an effect, if readers of this topic were to take action, they may begin to see the value in offering such blood tests!

So good readers, why not contact Life Extension and ask why they aren't offering telomere length lab measurments - even saliva telomere testing?

Here is the link I used http://www.lef.org/quest-com (there is a contact/email button at the bottom, but you may have to be a member. Worth tying to send the email question, even if you are not already a member.)

Thanks to all crowd participants in advance!
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Re: Telomere Timebomb

Post Number:#44  Post by Johnwen » Sat Jan 03, 2015 12:51 am

Owen;
Yep! I noticed this about LEF they used to be a good source but last couple of years it became apparent their moving towards the light of Pharma’s Money$$!!

So since it’s obvious by this posting your studying senescence (ageing & cell ageing) I figured maybe I should give you a little closer look at some of the other aspects. Things like the Hayflick Limit or premature senescence, replicative senescence all need to be considered as while as those diploid fibroblasts.
Here’s some links to wet your whistle!

http://rutchem.rutgers.edu/~kyc/pdf/2002%20last.pdf

WIKI:

http://en.wikipedia.org/wiki/WI-38


Check out the Hayflick limit and then at the upper part of the page the drop down menu on “Biology of Ageing.”

http://www.senescence.info/cell_aging.html

Then there’s this supplement that of course won’t do nothing but make expensive urine no matter what tests and studies say! Because it’s a supplement and won’t put money in Big Pharma’s Pocket$$$$

WIKI:

http://en.wikipedia.org/wiki/Carnosine

http://www.antiaging-systems.com/69-carnosine

http://carnosineelite.com/


Hope this helps in the quest for TRUE KNOWLEDGE!!!
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Re: Telomere Timebomb

Post Number:#45  Post by ofonorow » Sun Jan 04, 2015 11:33 am

Thanks again johnwen!

Speaking of knowledge, I am in the middle of Blackburn's book Telomeres (http://www.amazon.com/Telomeres-Cold-Spring-Harbor-Monograph/dp/0879698101/ref=sr_1_3?ie=UTF8&qid=1420392696&sr=8-3&keywords=telomeres+blackburn) This book is not for those trying to get their feet wet, rather it is by the primary researcher aimed at other researchers and classrooms. As Spock might say, Fascinating!
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